Gammalinolenic acid, an unsaturated fatty acid with anti-inflammatory properties, blocks amplification of IL-1 beta production by human monocytes
UMass Chan Affiliations
Department of Medicine, Division of RheumatologyDocument Type
Journal ArticlePublication Date
2001-06-22Keywords
Anti-Inflammatory Agents, Non-SteroidalArthritis, Rheumatoid
Cell Separation
Cells, Cultured
Dose-Response Relationship, Drug
Dose-Response Relationship, Immunologic
Female
Gene Expression Regulation
Humans
Interleukin-1
Lipopolysaccharides
Macrophage Activation
Middle Aged
Monocytes
Protein Precursors
RNA, Messenger
gamma-Linolenic Acid
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Administration of gammalinolenic acid (GLA), an unsaturated fatty acid, reduces joint inflammation in patients with rheumatoid arthritis. Addition of GLA in vitro suppresses release of IL-1beta from human monocytes stimulated with LPS. LPS-induced IL-1beta release is followed by IL-1-induced IL-1beta release, an amplification process termed autoinduction. We show here with peripheral blood monocytes from normal volunteers and from patients with rheumatoid arthritis by using IL-1R antagonist to block autoinduction and IL-1alpha stimulation to simulate autoinduction that approximately 40% of IL-1beta released from LPS-stimulated cells is attributable to autoinduction and that GLA reduces autoinduction of IL-1beta while leaving the initial IL-1beta response to LPS intact. Experiments with cells in which transcription and protein synthesis were blocked suggest that GLA induces a protein that reduces pro-IL-1beta mRNA stability. IL-1beta is important to host defense, but the amplification mechanism may be excessive in genetically predisposed patients. Thus, reduction of IL-1beta autoinduction may be protective in some patients with endotoxic shock and with diseases characterized by chronic inflammation.Source
J Immunol. 2001 Jul 1;167(1):490-6.
DOI
10.4049/jimmunol.167.1.490Permanent Link to this Item
http://hdl.handle.net/20.500.14038/38252PubMed ID
11418687Related Resources
ae974a485f413a2113503eed53cd6c53
10.4049/jimmunol.167.1.490