TLR activation synergizes with Kilham rat virus infection to induce diabetes in BBDR rats
Department of Medicine, Division of Infectious Diseases and Immunology; Department of Medicine, Division of Diabetes; Department of Medicine, Division of Endocrinology and Metabolism
Animals; Blotting, Western; Diabetes Mellitus, Experimental; Enzyme-Linked Immunosorbent Assay; Interferon Type II; Interleukins; Membrane Glycoproteins; Oxidoreductases; Parvoviridae; Parvoviridae Infections; Parvovirus; Poly I-C; RNA, Messenger; Rats; Receptors, Cell Surface; Reverse Transcriptase Polymerase Chain Reaction; Toll-Like Receptor 3; Toll-Like Receptors; Vaccinia; Vaccinia virus
Immunology and Infectious Disease | Life Sciences | Medicine and Health Sciences
Virus infection is hypothesized to be an important environmental "trigger" of type 1 diabetes in humans. We used the BBDR rat model to investigate the relationship between viral infection and autoimmune diabetes. BBDR rats are diabetes-free in viral Ab-free housing, but the disease develops in approximately 30% of BBDR rats infected with Kilham rat virus (KRV) through a process that does not involve infection of pancreatic beta cells. Pretreatment with polyinosinic-polycytidylic (poly(I:C)), a ligand of TLR3, acts synergistically to induce diabetes in 100% of KRV-infected rats. The mechanisms by which KRV induces diabetes and TLR3 ligation facilitates this process are not clear. In this study, we demonstrate that activation of the innate immune system plays a crucial role in diabetes induction. We report that multiple TLR agonists synergize with KRV infection to induce diabetes in BBDR rats, as do heat-killed Escherichia coli or Staphylococcus aureus (natural TLR agonists). KRV infection increases serum IL-12 p40 in a strain-specific manner, and increases IL-12 p40, IFN-gamma-inducible protein-10, and IFN-gamma mRNA transcript levels, particularly in the pancreatic lymph nodes of BBDR rats. Infection with vaccinia virus or H-1 parvovirus induced less stimulation of the innate immune system and failed to induce diabetes in BBDR rats. Our results suggest that the degree to which the innate immune system is activated by TLRs is important for expression of virus-induced diabetes in genetically susceptible hosts.
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Citation: J Immunol. 2005 Jan 1;174(1):131-42.
Zipris, Danny; Lien, Egil; Xie, Jenny X.; Greiner, Dale L.; Mordes, John P.; and Rossini, Aldo A., "TLR activation synergizes with Kilham rat virus infection to induce diabetes in BBDR rats" (2005). Open Access Articles. Paper 1099.