Identification and analysis for cross-reactivity among hantaviruses of H-2b-restricted cytotoxic T-lymphocyte epitopes in Sin Nombre virus nucleocapsid protein
Center for Infectious Disease and Vaccine Research
Amino Acid Sequence; Animals; Antibody Specificity; Cell Line, Tumor; Cross Reactions; Epitopes; H-2 Antigens; Hantavirus; Hantavirus Pulmonary Syndrome; Humans; Isoantigens; Major Histocompatibility Complex; Mice; Molecular Sequence Data; Nucleocapsid; Nucleocapsid Proteins; Peptide Fragments; Sin Nombre virus; T-Lymphocytes, Cytotoxic
Life Sciences | Medicine and Health Sciences
Sin Nombre virus (SNV) causes hantavirus pulmonary syndrome (HPS), with a high rate of mortality in humans who are infected by the transmission of virus from the natural rodent host. In humans, cytotoxic T lymphocytes (CTL) specific for SNV appear to play an important role in the pathogenicity of HPS. There is a correlation between the frequencies of SNV-specific CTLs and the severity of HPS disease. In order to create a mouse model to study the role of SNV-specific T cells in vivo, T cell responses to SNV nucleocapsid (N) protein in B6.PL Thy1(a)/Cy mice (H-2(b)) immunized with plasmid DNA or recombinant vaccinia virus expressing SNV N protein were examined. Four peptides, NC94-101, NC175-189, NC217-231 and NC331-345, were recognized by CD8(+) T cells in CTL and ELISPOT assays in SNV N-immunized mice. Interestingly, two of these epitopes are located in the central region of the SNV N protein, where several human CD8(+) T-cell epitopes have been defined in Puumala virus and SNV. CTL lines specific for these four epitopes were cross-reactive to corresponding Puumala virus peptides, but only one of them was cross-reactive to Hantaan virus peptides. These results will enable the analysis of the roles of CTL in immunopathology of HPS in experimental mouse models of HPS.
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Citation: J Gen Virol. 2004 Jul;85(Pt 7):1909-19. Link to article on publisher's site
DOI of Published Version
The Journal of general virology
Maeda, Ken; West, Kim; Toyosaki-Maeda, Tomoko; Rothman, Alan L.; Ennis, Francis A.; and Terajima, Masanori, "Identification and analysis for cross-reactivity among hantaviruses of H-2b-restricted cytotoxic T-lymphocyte epitopes in Sin Nombre virus nucleocapsid protein" (2004). Open Access Articles. 1071.