Title

Identification and analysis for cross-reactivity among hantaviruses of H-2b-restricted cytotoxic T-lymphocyte epitopes in Sin Nombre virus nucleocapsid protein

UMMS Affiliation

Center for Infectious Disease and Vaccine Research

Date

6-26-2004

Document Type

Article

Subjects

Amino Acid Sequence; Animals; Antibody Specificity; Cell Line, Tumor; Cross Reactions; Epitopes; H-2 Antigens; Hantavirus; Hantavirus Pulmonary Syndrome; Humans; Isoantigens; Major Histocompatibility Complex; Mice; Molecular Sequence Data; Nucleocapsid; Nucleocapsid Proteins; Peptide Fragments; Sin Nombre virus; T-Lymphocytes, Cytotoxic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Sin Nombre virus (SNV) causes hantavirus pulmonary syndrome (HPS), with a high rate of mortality in humans who are infected by the transmission of virus from the natural rodent host. In humans, cytotoxic T lymphocytes (CTL) specific for SNV appear to play an important role in the pathogenicity of HPS. There is a correlation between the frequencies of SNV-specific CTLs and the severity of HPS disease. In order to create a mouse model to study the role of SNV-specific T cells in vivo, T cell responses to SNV nucleocapsid (N) protein in B6.PL Thy1(a)/Cy mice (H-2(b)) immunized with plasmid DNA or recombinant vaccinia virus expressing SNV N protein were examined. Four peptides, NC94-101, NC175-189, NC217-231 and NC331-345, were recognized by CD8(+) T cells in CTL and ELISPOT assays in SNV N-immunized mice. Interestingly, two of these epitopes are located in the central region of the SNV N protein, where several human CD8(+) T-cell epitopes have been defined in Puumala virus and SNV. CTL lines specific for these four epitopes were cross-reactive to corresponding Puumala virus peptides, but only one of them was cross-reactive to Hantaan virus peptides. These results will enable the analysis of the roles of CTL in immunopathology of HPS in experimental mouse models of HPS.

Rights and Permissions

Citation: J Gen Virol. 2004 Jul;85(Pt 7):1909-19. Link to article on publisher's site

DOI of Published Version

10.1099/vir.0.79945-0

Related Resources

Link to Article in PubMed

Journal Title

The Journal of general virology

PubMed ID

15218176