Title
Cells capable of bone production engraft from whole bone marrow transplants in nonablated mice
PubMed ID
9989988
UMMS Affiliation
Cancer Center and the Department of Cell Biology
Date
2-17-1999
Document Type
Article
Subjects
Animals; Bone Marrow Cells; *Bone Marrow Transplantation; *Chimera; Female; Graft Survival; In Situ Hybridization, Fluorescence; Male; Mice; Mice, Inbred BALB C; Osteoblasts; *Osteogenesis; Stromal Cells; Y Chromosome
Disciplines
Medical Cell Biology | Oncology
Abstract
Allogeneic and autologous marrow transplants are routinely used to correct a wide variety of diseases. In addition, autologous marrow transplants potentially provide opportune means of delivering genes in transfected, engrafting stem cells. However, relatively little is known about the mechanisms of engraftment in transplant recipients, especially in the nonablated setting and with regard to cells not of hemopoietic origin. In particular, this includes stromal cells and progenitors of the osteoblastic lineage. We have demonstrated for the first time that a whole bone marrow transplant contains cells that engraft and become competent osteoblasts capable of producing bone matrix. This was done at the individual cell level in situ, with significant numbers of donor cells being detected by fluorescence in situ hybridization in whole femoral sections. Engrafted cells were functionally active as osteoblasts producing bone before being encapsulated within the bone lacunae and terminally differentiating into osteocytes. Transplanted cells were also detected as flattened bone lining cells on the periosteal bone surface.
Rights and Permissions
Citation: J Exp Med. 1999 Feb 15;189(4):729-34.