Bone marrow-derived antigen-presenting cells are required for the generation of cytotoxic T lymphocyte responses to viruses and use transporter associated with antigen presentation (TAP)-dependent and -independent pathways of antigen presentation
Department of Pathology
Animals; Antigen-Presenting Cells; Bone Marrow Cells; Bone Transplantation; Crosses, Genetic; Cytotoxicity, Immunologic; Lymphocytic choriomeningitis virus; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Poliovirus; T-Lymphocytes, Cytotoxic; *Transplantation Chimera; Tumor Cells, Cultured; Vaccinia virus
Medical Pathology | Medical Sciences
Bone marrow (BM)-derived professional antigen-presenting cells (pAPCs) are required for the generation of cytotoxic T lymphocyte (CTL) responses to vaccinia virus and poliovirus. Furthermore, these BM-derived pAPCs require a functional transporter associated with antigen presentation (TAP). In this report we analyze the requirements for BM-derived pAPCs and TAP in the initiation of CTL responses to lymphocytic choriomeningitis virus (LCMV) and influenza virus (Flu). Our results indicate a requirement for BM-derived pAPCs for the CTL responses to these viruses. However, we found that the generation of CTLs to one LCMV epitope (LCMV nucleoprotein 396-404) was dependent on BM-derived pAPCs but, surprisingly, TAP independent. The study of the CTL response to Flu confirmed the existence of this BM-derived pAPC-dependent/TAP-independent CTL response and indicated that the TAP-independent pathway is approximately 10-300-fold less efficient than the TAP-dependent pathway.
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Citation: J Exp Med. 2000 Oct 16;192(8):1143-50.