UMMS Affiliation

Division of Infectious Diseases and Immunology

Date

9-1-1995

Document Type

Article

Subjects

Antibodies, Monoclonal; Antigens, Viral; Enzyme-Linked Immunosorbent Assay; Feces; Humans; Norwalk virus

Disciplines

Infectious Disease | Medical Immunology

Abstract

Monoclonal antibodies against the prototype 8FIIa strain of Norwalk virus were prepared and applied to an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. The monoclonal antibodies immunoprecipitated a 58-kDa protein which had been produced by in vitro transcription-translation of Norwalk virus cloned cDNA, and they reacted by EIA with recombinant Norwalk virus capsid protein at a sensitivity level of 1 ng/ml. The EIA detected virus in all tested samples from 15 different Norwalk virus-infected volunteers. No cross-reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses.

Rights and Permissions

Citation: J Clin Microbiol. 1995 Sep;33(9):2511-3.

Related Resources

Link to Article in PubMed

Journal Title

Journal of clinical microbiology

PubMed ID

7494063

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