Division of Infectious Diseases and Immunology
Antibodies, Monoclonal; Antigens, Viral; Enzyme-Linked Immunosorbent Assay; Feces; Humans; Norwalk virus
Infectious Disease | Medical Immunology
Monoclonal antibodies against the prototype 8FIIa strain of Norwalk virus were prepared and applied to an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. The monoclonal antibodies immunoprecipitated a 58-kDa protein which had been produced by in vitro transcription-translation of Norwalk virus cloned cDNA, and they reacted by EIA with recombinant Norwalk virus capsid protein at a sensitivity level of 1 ng/ml. The EIA detected virus in all tested samples from 15 different Norwalk virus-infected volunteers. No cross-reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses.
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Citation: J Clin Microbiol. 1995 Sep;33(9):2511-3.
Journal of clinical microbiology
Herrmann, John E.; Blacklow, Neil R.; Matsui, Suzanne M.; Lewis, Terry L.; Estes, Mary K.; Ball, Judith M.; and Brinker, James P., "Monoclonal antibodies for detection of Norwalk virus antigen in stools" (1995). Open Access Articles. 1019.