University of Massachusetts Medical Center
Bacterial Typing Techniques
Blood culture records from 1994 to 1995 from five U.S. medical centers all using the Difco ESP continuous monitoring blood culture system were reviewed retrospectively. Among a total of 7,362 isolates of bacteria and yeasts, only 0.1% of possibly significant isolates would have been missed had blood cultures been routinely incubated for 4 days instead of the 5 days recommended by the manufacturer. Conversely, numerous contaminants, detected only on day 5, would have been eliminated by a 4-day incubation period.
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Citation: J Clin Microbiol. 1997 May;35(5):1290-2.
Journal of clinical microbiology
Doern, Gary V.; Brueggemann, Angela B.; Dunne, W. Michael; Jenkins, Stephen G.; Halstead, Diane C.; and McLaughlin, James C., "Four-day incubation period for blood culture bottles processed with the Difco ESP blood culture system" (1997). Open Access Articles. 1016.