UMMS Affiliation

Division of Infectious Diseases and Immunology

Date

8-17-1999

Document Type

Article

Subjects

Adolescent; Adult; Animals; Antibodies, Viral; Caliciviridae Infections; Child; Child, Preschool; Humans; Immunoglobulin M; Infant; Mice; Mice, Inbred BALB C; Middle Aged; Norwalk virus

Disciplines

Immunology and Infectious Disease

Abstract

Sera obtained from adult volunteers inoculated with genogroup II Norwalk-like viruses (NLVs), Hawaii virus, and Snow Mountain virus and from patients involved in outbreaks of gastroenteritis were tested for genogroup II NLV Mexico virus-specific immunoglobulin M (IgM) by use of a monoclonal antibody, recombinant Mexico virus antigen (rMXV)-based IgM capture enzyme-linked immunosorbent assay (ELISA). Sera from genogroup I Norwalk virus (NV)-inoculated volunteers and from patients involved in a genogroup I NLV outbreak were also tested. In sera from those infected with genogroup I NV or NLVs in volunteer and outbreak studies, only 3 of 25 were rMXV IgM positive; in contrast, 24 of 25 were IgM positive for recombinant NV (rNV). In sera from those infected with genogroup II NLVs in volunteer and outbreak studies, 28 of 47 were rMXV IgM positive and none were IgM positive for rNV, showing the specificity of each IgM test for its respective genogroup. In an outbreak of gastroenteritis not characterized as being of viral etiology but suspected to be due to NV, 7 of 13 persons had IgM responses to rMXV, whereas none had IgM responses to rNV, thus establishing the diagnosis as genogroup II NLV infection. The rMXV-based IgM capture ELISA developed is specific for the diagnosis of genogroup II NLV infections.

Rights and Permissions

Citation: J Clin Microbiol. 1999 Sep;37(9):2983-6.

Related Resources

Link to Article in PubMed

PubMed ID

10449486

 
 

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