UMMS Affiliation

Department of Pediatrics

Publication Date

6-1-1988

Document Type

Article

Subjects

Antibodies, Monoclonal; Blood Platelets; *Blood Preservation; Centrifugation; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Humans; Platelet Aggregation Inhibitors; Platelet Count; *Platelet Glycoprotein GPIb-IX Complex; Platelet Membrane Glycoproteins; Time Factors

Disciplines

Hematology | Pediatrics

Abstract

Platelet membrane glycoprotein (GP) Ib contains receptor for von Willebrand factor and thrombin. Its proteolytic fragment, glycocalicin, circulates in normal plasma. In this study, storage of platelet concentrates for 5 d resulted in a 221% increase in plasma glycocalicin (1.3 times the total amount of glycocalicin present on the surface of all platelets), an 8% overall increase in platelet surface GPIb, and the appearance of a surface GPIb-negative subpopulation of platelets. Total platelet GPIb content of fresh washed platelets, determined by gel electrophoresis and immunoassay of Triton X-100 lysates, averaged 159,740 molecules per platelet. There were 36,360 surface GPIb molecules per platelet, determined by immunoassay of the supernatant of fresh washed platelets whose surface GPIb had been completely plasmin-cleaved. In summary, these studies provide evidence for (a) a redistribution of GPIb molecules with platelet storage, and (b) a large intraplatelet pool of GPIb (approximately threefold larger than the platelet surface pool of GPIb).

Rights and Permissions

Citation: J Clin Invest. 1988 Jun;81(6):1734-40. Link to article on publisher's site

DOI of Published Version

10.1172/JCI113513

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

The Journal of clinical investigation

PubMed ID

3384948

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.