Expression and regulation of mPer1 in immortalized GnRH neurons
Department of Neurobiology
Medical Subject Headings
Animals; Cell Cycle Proteins; Cells, Cultured; Forskolin; Gene Expression Regulation; Gonadotropin-Releasing Hormone; Immunoblotting; Immunohistochemistry; Mice; Neurons; Neuroprotective Agents; Nuclear Proteins; Period Circadian Proteins; Preoptic Area; Reverse Transcriptase Polymerase Chain Reaction; Vasoactive Intestinal Peptide
Neuroscience and Neurobiology
Hypothalamic GnRH (gonadotropin-releasing hormone) neurons play a critical role in the initiation and maintenance of reproduction competence. Using the mouse GnRH neuronal cell line, GT1-7, we have characterized the expression of the gene mPer1, a recognized key element of the mammalian circadian clockwork. Both mPer1 transcripts and the 136 kDa mPER1 gene product could be detected in these cells. Immunocytochemical analysis also confirmed expression of mPER1 both in vitro and in vivo in GnRH neurons. Activation of cyclic AMP signalling pathways in vitro elevated GnRH secretion as well as mPer1 expression and nuclear mPER1 immunoreactivity. As mPER1 is known to feedback on transcriptional activities in many cell models, the data presented here point to a role for mPER1 in the regulation of gene expression in GnRH neurons, and thus in the control of neuroendocrine activities.
Rights and Permissions
Citation: Neuroreport. 2003 Mar 24;14(4):613-8. Link to article on publisher's site