Document Type
Journal ArticlePublication Date
2003-03-24Keywords
AnimalsCell Cycle Proteins
Cells, Cultured
Forskolin
Gene Expression Regulation
Gonadotropin-Releasing Hormone
Immunoblotting
Immunohistochemistry
Mice
Neurons
Neuroprotective Agents
Nuclear Proteins
Period Circadian Proteins
Preoptic Area
Reverse Transcriptase Polymerase Chain Reaction
Vasoactive Intestinal Peptide
Neuroscience and Neurobiology
Metadata
Show full item recordAbstract
Hypothalamic GnRH (gonadotropin-releasing hormone) neurons play a critical role in the initiation and maintenance of reproduction competence. Using the mouse GnRH neuronal cell line, GT1-7, we have characterized the expression of the gene mPer1, a recognized key element of the mammalian circadian clockwork. Both mPer1 transcripts and the 136 kDa mPER1 gene product could be detected in these cells. Immunocytochemical analysis also confirmed expression of mPER1 both in vitro and in vivo in GnRH neurons. Activation of cyclic AMP signalling pathways in vitro elevated GnRH secretion as well as mPer1 expression and nuclear mPER1 immunoreactivity. As mPER1 is known to feedback on transcriptional activities in many cell models, the data presented here point to a role for mPER1 in the regulation of gene expression in GnRH neurons, and thus in the control of neuroendocrine activities.Source
Neuroreport. 2003 Mar 24;14(4):613-8. Link to article on publisher's siteDOI
10.1097/01.wnr.0000063508.18654.3cPermanent Link to this Item
http://hdl.handle.net/20.500.14038/38052PubMed ID
12657897Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1097/01.wnr.0000063508.18654.3c