Mutagenesis with Drosophila
Department of Neurobiology
Medical Subject Headings
Animals; Circadian Rhythm; Crosses, Genetic; Drosophila; Ethyl Methanesulfonate; Female; *Genes, Insect; Male; *Mutagenesis; Mutagenesis, Insertional; Mutagens; X Chromosome
Neuroscience and Neurobiology
Forward genetics in Drosophila has profoundly affected our understanding of circadian rhythms in this organism and, more generally, in the animal kingdom. Most Drosophila pacemaker genes were discovered through the isolation of gene variants affecting the free-running period of the circadian pacemaker. There are different ways to mutagenize flies. An alkylating agent can be used to randomly alter the fly genome, or transposable elements can be mobilized to disrupt or increase the expression of the targeted genes. The advantages of these different methods are complementary.
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Citation: Methods Mol Biol. 2007;362:187-95. DOI 10.1007/978-1-59745-257-1_13