Title
RNase protection assay
UMMS Affiliation
Department of Neurobiology
Date
2-2-2007
Document Type
Book Chapter
Medical Subject Headings
Alternative Splicing; Antisense Elements (Genetics); Bacteriophage T7; Circadian Rhythm; DNA-Directed RNA Polymerases; Molecular Probe Techniques; Promoter Regions, Genetic; RNA, Messenger; RNA, Viral; *Ribonucleases; Viral Proteins
Disciplines
Neuroscience and Neurobiology
Abstract
The RNase protection assay is a standard approach to determine mRNA levels of a gene of interest in different tissues, developmental stages, or times of the day. Splicing or promoter variants can be studied with specific probes. It is widely used in chronobiology to study the temporal profile of expression of circadian genes and the effects of genetic manipulation on these oscillations. Methods to generate the riboprobes and to perform the RNase protection assay itself are described in this chapter.
Rights and Permissions
Citation: Methods Mol Biol. 2007;362:343-8. DOI 10.1007/978-1-59745-257-1_24