Title
Protein extraction from Drosophila heads
UMMS Affiliation
Department of Neurobiology
Date
2-2-2007
Document Type
Book Chapter
Medical Subject Headings
Animals; Blotting, Western; Circadian Rhythm; Drosophila; Drosophila Proteins; Head; Immunoprecipitation; Phosphorylation; Photoperiod; Temperature
Disciplines
Neuroscience and Neurobiology
Abstract
In Drosophila, the concentration and phosphorylation levels of several important circadian proteins (e.g., PERIOD, TIMELESS) oscillate on a 24-h basis. A simple and rapid method for extracting proteins from fly heads is presented here. The extracts can immediately be loaded onto an sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel to assay the effects of mutations, genetic manipulations, or environmental conditions on the oscillations of circadian proteins by Western blotting. They can also be used for immunoprecipitation experiments.
Rights and Permissions
Citation: Methods Mol Biol. 2007;362:375-7. DOI 10.1007/978-1-59745-257-1_27