Title

Protein extraction from Drosophila heads

UMMS Affiliation

Department of Neurobiology; Emery Lab

Date

2-2-2007

Document Type

Book Chapter

Medical Subject Headings

Animals; Blotting, Western; Circadian Rhythm; Drosophila; Drosophila Proteins; Head; Immunoprecipitation; Phosphorylation; Photoperiod; Temperature

Disciplines

Neuroscience and Neurobiology

Abstract

In Drosophila, the concentration and phosphorylation levels of several important circadian proteins (e.g., PERIOD, TIMELESS) oscillate on a 24-h basis. A simple and rapid method for extracting proteins from fly heads is presented here. The extracts can immediately be loaded onto an sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel to assay the effects of mutations, genetic manipulations, or environmental conditions on the oscillations of circadian proteins by Western blotting. They can also be used for immunoprecipitation experiments.

Rights and Permissions

Citation: Methods Mol Biol. 2007;362:375-7. DOI 10.1007/978-1-59745-257-1_27

Related Resources

Link to Article in PubMed

PubMed ID

17417024