Title

Formation and function of flavin anion radical in cryptochrome 1 blue-light photoreceptor of monarch butterfly

UMMS Affiliation

Department of Neurobiology; Reppert Lab

Date

6-15-2007

Document Type

Article

Medical Subject Headings

Animals; Anions; *Butterflies; Cryptochromes; Flavins; Flavoproteins; Insect Proteins; Oxidation-Reduction; Photochemistry; Photoreceptor Cells, Invertebrate

Disciplines

Neuroscience and Neurobiology

Abstract

The monarch butterfly (Danaus plexippus) cryptochrome 1 (DpCry1) belongs in the class of photosensitive insect cryptochromes. Here we purified DpCry1 expressed in a bacterial host and obtained the protein with a stoichiometric amount of the flavin cofactor in the two-electron oxidized, FAD(ox), form. Exposure of the purified protein to light converts the FAD(ox) to the FAD*(-) flavin anion radical by intraprotein electron transfer from a Trp residue in the apoenzyme. To test whether this novel photoreduction reaction is part of the DpCry1 physiological photocycle, we mutated the Trp residue that acts as the ultimate electron donor in flavin photoreduction. The mutation, W328F, blocked the photoreduction entirely but had no measurable effect on the light-induced degradation of DpCry1 in vivo. In light of this finding and the recently published action spectrum of this class of Crys, we conclude that DpCry1 and similar insect cryptochromes do not contain flavin in the FAD(ox) form in vivo and that, most likely, the [see text] photoreduction reaction is not part of the insect cryptochrome photoreaction that results in proteolytic degradation of the photopigment.

Rights and Permissions

Citation: J Biol Chem. 2007 Jun 15;282(24):17608-12. Epub 2007 Apr 25. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

17459876