Title

Nucleus to Synapse Nesprin1 Railroad Tracks Direct Synapse Maturation through RNA Localization

UMMS Affiliation

Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

5-20-2015

Document Type

Article

Medical Subject Headings

Actins; Animals; Cell Nucleus; Drosophila; Drosophila Proteins; Microfilament Proteins; Muscle Proteins; Neuromuscular Junction; Organogenesis; RNA; Signal Transduction; Synapses

Disciplines

Developmental Neuroscience | Molecular and Cellular Neuroscience

Abstract

An important mechanism underlying synapse development and plasticity is the localization of mRNAs that travel from the nucleus to synaptic sites. Here we demonstrate that the giant nuclear-associated Nesprin1 (dNesp1) forms striated F-actin-based filaments, which we dubbed "railroad tracks," that span from muscle nuclei to postsynaptic sites at the neuromuscular junction in Drosophila. These railroad tracks specifically wrap around immature boutons formed during development and in response to electrical activity. In the absence of dNesp1, mRNAs normally localized at postsynaptic sites are lacking and synaptic maturation is inhibited. This dNesp1 function does not depend on direct association of dNesp1 isoforms with the nuclear envelope. We also show that dNesp1 functions with an unconventional myosin, Myo1D, and that both dNesp1 and Myo1D are mutually required for their localization to immature boutons. These studies unravel a novel pathway directing the transport of mRNAs from the nucleus to postsynaptic sites during synaptic maturation.

Rights and Permissions

Citation: Neuron. 2015 May 20;86(4):1015-28. doi: 10.1016/j.neuron.2015.04.006. Epub 2015 May 7. Link to article on publisher's site

Comments

This article was recommended as Exceptional in an F1000Prime review by Faculty of 1000.

Co-author Vahbiz Jokhi is a doctoral student in the Neuroscience program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Related Resources

Link to Article in PubMed

PubMed ID

25959729