Nucleus to Synapse Nesprin1 Railroad Tracks Direct Synapse Maturation through RNA Localization
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Student Authors
James AshleyVahbiz Jokhi
Academic Program
NeuroscienceUMass Chan Affiliations
Graduate School of Biomedical Sciences, Neuroscience ProgramBudnik Lab
Neurobiology
Document Type
Journal ArticlePublication Date
2015-05-20Keywords
ActinsAnimals
Cell Nucleus
Drosophila
Drosophila Proteins
Microfilament Proteins
Muscle Proteins
Neuromuscular Junction
Organogenesis
RNA
Signal Transduction
Synapses
Developmental Neuroscience
Molecular and Cellular Neuroscience
Metadata
Show full item recordAbstract
An important mechanism underlying synapse development and plasticity is the localization of mRNAs that travel from the nucleus to synaptic sites. Here we demonstrate that the giant nuclear-associated Nesprin1 (dNesp1) forms striated F-actin-based filaments, which we dubbed "railroad tracks," that span from muscle nuclei to postsynaptic sites at the neuromuscular junction in Drosophila. These railroad tracks specifically wrap around immature boutons formed during development and in response to electrical activity. In the absence of dNesp1, mRNAs normally localized at postsynaptic sites are lacking and synaptic maturation is inhibited. This dNesp1 function does not depend on direct association of dNesp1 isoforms with the nuclear envelope. We also show that dNesp1 functions with an unconventional myosin, Myo1D, and that both dNesp1 and Myo1D are mutually required for their localization to immature boutons. These studies unravel a novel pathway directing the transport of mRNAs from the nucleus to postsynaptic sites during synaptic maturation.Source
Neuron. 2015 May 20;86(4):1015-28. doi: 10.1016/j.neuron.2015.04.006. Epub 2015 May 7. Link to article on publisher's siteDOI
10.1016/j.neuron.2015.04.006Permanent Link to this Item
http://hdl.handle.net/20.500.14038/37876PubMed ID
25959729Notes
This article was recommended as Exceptional in an F1000Prime review by Faculty of 1000. Co-author Vahbiz Jokhi is a doctoral student in the Neuroscience program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.neuron.2015.04.006