Protein Gq modulates termination of phototransduction and prevents retinal degeneration
Student Authors
Peiyi GuoAcademic Program
NeuroscienceUMass Chan Affiliations
Graduate School of Biomedical Sciences, Neuroscience ProgramLi Lab
Neurobiology
Document Type
Journal ArticlePublication Date
2012-04-20Keywords
AllelesAnimals
Animals, Genetically Modified
Arrestins
Drosophila Proteins
Drosophila melanogaster
Electrophysiology
GTP-Binding Protein alpha Subunits, Gq-G11
Light
Light Signal Transduction
Models, Genetic
Mutation
Photoreceptor Cells, Invertebrate
Receptors, G-Protein-Coupled
Retinal Degeneration
Rhodopsin
Biochemistry, Biophysics, and Structural Biology
Neuroscience and Neurobiology
Metadata
Show full item recordAbstract
Appropriate termination of the phototransduction cascade is critical for photoreceptors to achieve high temporal resolution and to prevent excessive Ca(2+)-induced cell toxicity. Using a genetic screen to identify defective photoresponse mutants in Drosophila, we isolated and identified a novel Galpha(q) mutant allele, which has defects in both activation and deactivation. We revealed that G(q) modulates the termination of the light response and that metarhodopsin/G(q) interaction affects subsequent arrestin-rhodopsin (Arr2-Rh1) binding, which mediates the deactivation of metarhodopsin. We further showed that the Galpha(q) mutant undergoes light-dependent retinal degeneration, which is due to the slow accumulation of stable Arr2-Rh1 complexes. Our study revealed the roles of G(q) in mediating photoresponse termination and in preventing retinal degeneration. This pathway may represent a general rapid feedback regulation of G protein-coupled receptor signaling.Source
J Biol Chem. 2012 Apr 20;287(17):13911-8. doi: 10.1074/jbc.M112.339895. Link to article on publisher's siteDOI
10.1074/jbc.M112.339895Permanent Link to this Item
http://hdl.handle.net/20.500.14038/37872PubMed ID
22389492Notes
Co-author Peiyi Guo is a doctoral student in the Neuroscience Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.
Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1074/jbc.M112.339895