Title

Dissection of Drosophila larval body-wall muscles

UMMS Affiliation

Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

8-1-2010

Document Type

Article

Medical Subject Headings

Animals; Dissection; Drosophila melanogaster; Larva; Magnetics; Muscles

Disciplines

Developmental Neuroscience | Molecular and Cellular Neuroscience

Abstract

Over the last two decades, the Drosophila larval neuromuscular junction has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. A number of different techniques have been used to dissect third-instar larval preparations to expose the body-wall muscles. Here, we describe a procedure that uses magnetic chambers and pins to allow for fine control in spreading the larval body wall.

Rights and Permissions

Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5469. doi: 10.1101/pdb.prot5469.

Comments

First author Preethi Ramachandran is a doctoral student in the Neuroscience Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Related Resources

Link to Article in PubMed

PubMed ID

20679378