Title

Immunocytochemical staining of Drosophila larval body-wall muscles

UMMS Affiliation

Department of Neurobiology

Date

8-1-2010

Document Type

Article

Medical Subject Headings

Animals; Dissection; Drosophila melanogaster; Immunohistochemistry; Larva; Muscles; Staining and Labeling

Disciplines

Neuroscience and Neurobiology

Abstract

Over the last two decades, the Drosophila larval neuromuscular junction (NMJ) has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. This article describes a procedure for labeling various proteins with antibodies in dissected larval body-wall muscles and visualizing their localization and distribution in the brain, NMJ, and muscle.

Rights and Permissions

Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5470. doi: 10.1101/pdb.prot5470.

Related Resources

Link to Article in PubMed