Immunocytochemical staining of Drosophila larval body-wall muscles

UMMS Affiliation

Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Publication Date


Document Type



Animals; Dissection; Drosophila melanogaster; Immunohistochemistry; Larva; Muscles; Staining and Labeling


Neuroscience and Neurobiology


Over the last two decades, the Drosophila larval neuromuscular junction (NMJ) has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. This article describes a procedure for labeling various proteins with antibodies in dissected larval body-wall muscles and visualizing their localization and distribution in the brain, NMJ, and muscle.

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Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5470. doi: 10.1101/pdb.prot5470.

Journal/Book/Conference Title

Cold Spring Harbor protocols


First author Preethi Ramachandran is a doctoral student in the Neuroscience Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Related Resources

Link to Article in PubMed

PubMed ID