Immunocytochemical staining of Drosophila larval body-wall muscles
Department of Neurobiology
Medical Subject Headings
Animals; Dissection; Drosophila melanogaster; Immunohistochemistry; Larva; Muscles; Staining and Labeling
Neuroscience and Neurobiology
Over the last two decades, the Drosophila larval neuromuscular junction (NMJ) has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. This article describes a procedure for labeling various proteins with antibodies in dissected larval body-wall muscles and visualizing their localization and distribution in the brain, NMJ, and muscle.
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Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5470. doi: 10.1101/pdb.prot5470.