UMMS Affiliation

Department of Neurobiology; Lois Lab

Date

6-7-2012

Document Type

Article

Medical Subject Headings

Enhancer Elements, Genetic; Neurons

Disciplines

Neuroscience and Neurobiology

Abstract

The ability to label, visualize, and manipulate subsets of neurons is critical for elucidating the structure and function of individual cell types in the brain. Enhancer trapping has proved extremely useful for the genetic manipulation of selective cell types in Drosophila. We have developed an enhancer trap strategy in mammals by generating transgenic mice with lentiviral vectors carrying single-copy enhancer-detector probes encoding either the marker gene lacZ or Cre recombinase. This transgenic strategy allowed us to genetically identify a wide variety of neuronal subpopulations in distinct brain regions. Enhancer detection by lentiviral transgenesis could thus provide a complementary method for generating transgenic mouse libraries for the genetic labeling and manipulation of neuronal subsets.

Comments

Citation: Kelsch W, Stolfi A, Lois C (2012) Genetic Labeling of Neuronal Subsets through Enhancer Trapping in Mice. PLoS ONE 7(6): e38593. doi:10.1371/journal.pone.0038593. Link to article on publisher's site

Copyright: © 2012 Kelsch et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Related Resources

Link to Article in PubMed

PubMed ID

22685588

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