Title

Diffusion-weighted magnetic resonance imaging: rapid and quantitative detection of focal brain ischemia

UMMS Affiliation

Department of Neurology; Department of Radiology; Graduate School of Biomedical Sciences

Date

1-1992

Document Type

Article

Subjects

Animals; Brain Ischemia; Cerebral Infarction; Diffusion; Evaluation Studies as Topic; Magnetic Resonance Imaging; Male; Rats; Rats, Inbred Strains; Staining and Labeling; Tetrazolium Salts; Time Factors

Disciplines

Neurology | Radiology

Abstract

We examined serial changes of diffusion- (DWI) and T2-weighted (T2WI) magnetic resonance images 30 minutes to 3 hours after intraluminal suture occlusion of the middle cerebral artery (MCA) in eight rats and after sham occlusion in four. We correlated the abnormal areas on DWI and T2WI with postmortem areas of infarction determined by 2,3,5-triphenyltetrazolium chloride (TTC), 24 hours after the operation. The 30-minute DWI in each MCA-occluded rat demonstrated increased signal intensity in the ipsilateral MCA territory, while T2WI showed no changes. At 3 hours, the ipsilateral DWI signal intensity increased further and the area of abnormality slightly increased. In some animals, the 3-hour T2WI disclosed an area of hyperintensity significantly smaller than that seen on the 30-minute DWI. TTC staining demonstrated an extensive MCA infarction in all rats with permanent MCA occlusion, confirmed by hematoxylin and eosin staining. The percent infarcted area of coronal brain sections, as determined by TTC staining, correlated significantly with areas on similar DWI sections at both 30 minutes and 3 hours. Sham-occluded control animals did not display any changes on DWI, T2WI, or TTC staining. The present study suggests that DWI is a very sensitive modality for detecting early ischemic brain injury, being highly correlated with post-mortem area of infarction, and may be useful to assess pharmacologic intervention.

Rights and Permissions

Citation: Neurology. 1992 Jan;42(1):235-40.

Related Resources

Link to article in PubMed

PubMed ID

1370863