Title
Embryonic stem cell rescue of tremor and ataxia in myelin-deficient shiverer mice
UMMS Affiliation
Department of Neurology; Department of Cell Biology
Date
1-2009
Document Type
Article
Subjects
Stem cells; Myelin basic protein; *X-gal histochemistry; Blastocyst injection; Chimera; Oligodendrocytes; Shiverer mice; White matter
Disciplines
Cell Biology | Neurology | Neuroscience and Neurobiology
Abstract
Transplantation of neural precursor cells has been proposed as a possible approach for replacing missing or damaged central nervous system myelin. Neonatal and adult myelin-deficient shiverer (shi) mice, bearing a mutation of the myelin basic protein (MBP) gene, have been used extensively as hosts for testing cell engraftment, migration, and myelination, but relatively little progress has been made in reversing shi motor deficits. Here we describe a prenatal cell replacement strategy, showing that embryonic stem cells injected into shi blastocyst embryos can generate chimeric mice with strong and widespread immunoreactive MBP expression throughout the brain and a behavioral (motor) phenotype that appears essentially rescued.
Rights and Permissions
Citation: J Neurol Sci. 2009 Jan 15;276(1-2):133-7. Epub 2008 Nov 8. Link to article on publisher's site
Related Resources
PubMed ID
18996543