Embryonic stem cell rescue of tremor and ataxia in myelin-deficient shiverer mice
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Authors
Low, Hoi PangGreco, Beatrice
Tanahashi, Yusuke
Gallant, Judith
Jones, Stephen N.
Billings-Gagliardi, Susan
Recht, Lawrence D.
Schwartz, William J.
Document Type
Journal ArticlePublication Date
2009-01-01Keywords
Stem cellsMyelin basic protein
*X-gal histochemistry
Blastocyst injection
Chimera
Oligodendrocytes
Shiverer mice
White matter
Cell Biology
Neurology
Neuroscience and Neurobiology
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Show full item recordAbstract
Transplantation of neural precursor cells has been proposed as a possible approach for replacing missing or damaged central nervous system myelin. Neonatal and adult myelin-deficient shiverer (shi) mice, bearing a mutation of the myelin basic protein (MBP) gene, have been used extensively as hosts for testing cell engraftment, migration, and myelination, but relatively little progress has been made in reversing shi motor deficits. Here we describe a prenatal cell replacement strategy, showing that embryonic stem cells injected into shi blastocyst embryos can generate chimeric mice with strong and widespread immunoreactive MBP expression throughout the brain and a behavioral (motor) phenotype that appears essentially rescued.Source
J Neurol Sci. 2009 Jan 15;276(1-2):133-7. Epub 2008 Nov 8. Link to article on publisher's siteDOI
10.1016/j.jns.2008.09.037Permanent Link to this Item
http://hdl.handle.net/20.500.14038/37649PubMed ID
18996543Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.jns.2008.09.037