Title

Inhibition of stimulated human leukocyte hydrogen peroxide generation by a novel antioxidant, OPC-14117

UMMS Affiliation

Department of Neurology

Date

5-1-1992

Document Type

Article

Subjects

Adult; Antioxidants; Depression, Chemical; Dose-Response Relationship, Drug; Female; *Free Radical Scavengers; Free Radicals; Humans; Hydrogen Peroxide; Indans; Leukocytes, Mononuclear; Male; Neutrophils; Piperazines; Respiratory Burst

Disciplines

Nervous System Diseases | Neurology | Pharmacy and Pharmaceutical Sciences

Abstract

Oxygen free radicals generated by leukocytes may contribute to tissue injury after central nervous system (CNS) focal ischemia or trauma. Inhibiting oxygen free radicals has improved outcome in experimental models of these conditions and antioxidant therapy appears promising. We evaluated the ability of a novel antioxidant, OPC-14117, to reduce hydrogen peroxide (H2O2) production by stimulated human polymorphonuclear (PMN) leukocytes and monocytes. Stimulated PMN and monocytes were incubated with several concentrations of OPC-14117 for 20 min and H2O2 production, nmol/1 x 10(6) cells/30 min, was measured. OPC-14117 significantly reduced PMN H2O2 production (P less than 0.001) and monocyte H2O2 production (P less than 0.05). A dose response relationship was observed for both leukocytes, as the 100 microM drug concentration was significantly (P less than 0.05) more effective than the 10 microM concentration. These results demonstrate that OPC-14117 inhibits H2O2 generation by stimulated human leukocytes and support further studies of its effects in disorders such as CNS focal ischemia and trauma, conditions where antioxidant therapy may be beneficial.

Rights and Permissions

Citation: J Neurol Sci. 1992 May;109(1):107-10.

Related Resources

Link to article in PubMed

PubMed ID

1517759