A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae
Department of Molecular Genetics and Microbiology
Medical Subject Headings
Base Sequence; Chromosome Mapping; Chromosomes, Bacterial; Genes, Bacterial; *Genome, Bacterial; Haemophilus influenzae; Mutagenesis, Insertional; Open Reading Frames; Phenotype; Plasmids
Microbiology | Molecular genetics
A high-density transposon mutagenesis strategy was applied to the Haemophilus influenzae genome to identify genes required for growth or viability. This analysis detected putative essential roles for the products of 259 ORFs of unknown function. Comparisons between complete genomes defined a subset of these proteins in H. influenzae having homologs in Mycobacterium tuberculosis that are absent in Saccharomyces cerevisiae, a distribution pattern that favors their use in development of antimicrobial therapeutics. Three genes within this set are essential for viability in other bacteria. Interfacing the set of essential gene products in H. influenzae with the distribution of homologs in other microorganisms can detect components of unrecognized cellular pathways essential in diverse bacteria. This genome-scale phenotypic analysis identifies potential roles for a large set of genes of unknown function.
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Citation: Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):966-71. Link to article on publisher's site