Title

Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; UMass Metabolic Network

Date

12-1-2016

Document Type

Article

Disciplines

Biochemistry | Cell Biology | Cellular and Molecular Physiology | Genomics | Molecular Biology

Abstract

We present Micro-C XL, an improved method for analysis of chromosome folding at mononucleosome resolution. Using long crosslinkers and isolation of insoluble chromatin, Micro-C XL increases signal-to-noise ratio. Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosome fiber features such as chromosomally interacting domains and higher order features such as centromere clustering. Micro-C XL provides a single assay to interrogate chromosome folding at length scales from the nucleosome to the full genome.

Rights and Permissions

Citation: Nat Methods. 2016 Dec;13(12):1009-1011. doi: 10.1038/nmeth.4025. Epub 2016 Oct 10. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

27723753