Department of Molecular, Cell and Cancer Biology
Letter to the Editor
Cancer Biology | Cell Biology | Cellular and Molecular Physiology | Genetics | Genomics | Hematology | Molecular Biology | Molecular Genetics | Oncology
We have now examined the erythroid phenotype in this zebrafish strain carrying a ZFN genomic knockout of piezo1. Genotyping was performed as previously described. In contrast to the anemic phenotype observed in zebrafish subjected to morpholino knockdown of piezo, the genomic ZFN knockout of piezo1 did not segregate either with anemia in the 3-dpf embryo or with dysmorphic erythrocyte morphology in the adult fish.
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Citation: Haematologica. 2015 Dec;100(12):e483-5. doi: 10.3324/haematol.2015.132449. Epub 2015 Aug 20. Link to article on publisher's site
PIEZO1, anemia, erythrocyte, morpholino oligomer, zebrafish, zinc-finger nuclease
Shmukler, Boris E.; Huston, Nicholas C.; Thon, Jonathan N.; Ni, Chih-Wen; Kourkoulis, George; Lawson, Nathan D.; Paw, Barry H.; and Alper, Seth L., "Homozygous knockout of the piezo1 gene in the zebrafish is not associated with anemia" (2015). Molecular, Cell and Cancer Biology Publications. 45.
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