Research resource: Monitoring endoplasmic reticulum membrane integrity in beta-cells at the single-cell level
Department of Molecular, Cell and Cancer Biology
Cell Biology | Cellular and Molecular Physiology | Endocrinology
Endoplasmic reticulum (ER) membrane integrity is an emerging target for human chronic diseases associated with ER stress. Despite the underlying importance of compromised ER membrane integrity in disease states, the entire process leading to ER membrane permeabilization and cell death is still not clear due to technical limitations. Here we describe a novel method for monitoring ER membrane integrity at the single-cell level in real time. Using a beta-cell line expressing ER-targeted redox sensitive green fluorescent protein, we could identify a beta-cell population undergoing ER membrane permeabilization induced by palmitate and could monitor cell fate and ER stress of these cells at the single-cell level. Our method could be used to develop a novel therapeutic modality targeting the ER membrane for ER-associated disorders, including beta-cell death in diabetes, neurodegeneration, and Wolfram syndrome.
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Citation: Mol Endocrinol. 2015 Mar;29(3):473-80. doi: 10.1210/me.2014-1260. Epub 2015 Jan 13. Link to article on publisher's site
Kanekura, Kohsuke; Ou, Jianhong; Hara, Takashi; Zhu, Lihua Julie; and Urano, Fumihiko, "Research resource: Monitoring endoplasmic reticulum membrane integrity in beta-cells at the single-cell level" (2015). Molecular, Cell and Cancer Biology Publications. 35.