MaPS Research Data

UMMS Affiliation

Department of Microbiology and Physiological Systems

Document Type

Data

File Format & Size

.xlsx (4 MB)

Publication Date

12-15-2015

Funders

National Institute of Allergy and Infectious Diseases, National Institutes of Health, U.S. Department of Health and Human Services

Grant Number

R01 AI106725

Keywords

T cells, Cytotoxic T cells, Memory T cells, Mycobacterium tuberculosis, Vaccination and immunization

Description

Manuscript abstract: T cell vaccines against Mycobacterium tuberculosis (Mtb) and other pathogens are based on the principle that memory T cells rapidly generate effector responses upon challenge, leading to pathogen clearance. Despite eliciting a robust memory CD8+ T cell response to the immunodominant Mtb antigen TB10.4 (EsxH), we find the increased frequency of TB10.4-specific CD8+ T cells conferred by vaccination to be short-lived after Mtb challenge. To compare memory and naïve CD8+ T cell function during their response to Mtb, we track their expansions using TB10.4-specific retrogenic CD8+ T cells. We find that the primary (naïve) response outnumbers the secondary (memory) response during Mtb challenge, an effect moderated by increased TCR affinity. To determine whether the expansion of polyclonal memory T cells is restrained following Mtb challenge, we used TCRβ deep sequencing to track TB10.4-specific CD8+ T cells after vaccination and subsequent challenge in intact mice. Successful memory T cells, defined by their clonal expansion after Mtb challenge, express similar CDR3β sequences suggesting TCR selection by antigen. Thus, both TCR-dependent and -independent factors affect the fitness of memory CD8+ responses. The impaired expansion of the majority of memory T cell clonotypes may explain why some TB vaccines have not provided better protection.

Data Collection Start Date

2014

Data Collection End Date

2015

Methodology

Methodology is documented in published manuscript.

Publisher

eScholarship@UMMS

Language

eng

Code Lists

See Additional File that contains an explanation of the data fields in the spreadsheet.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

immunoSEQ_Codel.pdf (224 kB)
Code list information for dataset