Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function
Authors
Mallappa, ChandrashekaraNasipak, Brian T.
Etheridge, Letitiah
Androphy, Elliot J.
Jones, Stephen N.
Sagerstrom, Charles G.
Ohkawa, Yasuyuki
Imbalzano, Anthony N.
UMass Chan Affiliations
Department of MedicineDepartment of Biochemistry and Molecular Pharmacology
Department of Cell Biology
Document Type
Journal ArticlePublication Date
2010-07-28Keywords
Adenosine TriphosphateAnimals
Cells, Cultured
Chromatin
*Chromatin Assembly and Disassembly
DNA Helicases
Gene Expression Regulation
Mice
Mice, Inbred C57BL
MicroRNAs
Muscle Development
Myoblasts
Nuclear Proteins
Oligonucleotides, Antisense
Ribonuclease III
Transcription Factors
Zebrafish
Zebrafish Proteins
Cell Biology
Metadata
Show full item recordAbstract
Knockdown of the Brg1 ATPase subunit of SWI/SNF chromatin remodeling enzymes in developing zebrafish caused stunted tail formation and altered sarcomeric actin organization, which phenocopies the loss of the microRNA processing enzyme Dicer, or the knockdown of myogenic microRNAs. Furthermore, myogenic microRNA expression and differentiation was blocked in Brg1 conditional myoblasts differentiated ex vivo. The binding of Brg1 upstream of myogenic microRNA sequences correlated with MyoD binding and accessible chromatin structure in satellite cells and myofibers, and it was required for chromatin accessibility and microRNA expression in a tissue culture model for myogenesis. The results implicate ATP-dependent chromatin remodelers in myogenic microRNA gene regulation.Source
Mol Cell Biol. 2010 Jul;30(13):3176-86. Epub 2010 Apr 26. Link to article on publisher's siteDOI
10.1128/MCB.00214-10Permanent Link to this Item
http://hdl.handle.net/20.500.14038/36032Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1128/MCB.00214-10