UMMS Affiliation

Department of Cell and Developmental Biology

Date

6-26-2013

Document Type

Article

Medical Subject Headings

Active Transport, Cell Nucleus; Amino Acid Sequence; Animals; Ataxia Telangiectasia Mutated Proteins; Cell Line, Tumor; Cell Nucleus; *DNA Damage; HCT116 Cells; Humans; Karyopherins; MicroRNAs; Molecular Sequence Data; Mutation; Nuclear Pore Complex Proteins; Phosphorylation; Proto-Oncogene Proteins c-akt; Sequence Homology, Amino Acid; Signal Transduction

Disciplines

Cell Biology | Molecular genetics

Abstract

Expression of microRNAs (miRNAs) involves transcription of miRNA genes and maturation of the primary transcripts. Recent studies have shown that posttranscriptional processing of primary and precursor miRNAs is induced after DNA damage through regulatory RNA-binding proteins in the Drosha and Dicer complexes, such as DDX5 and KSRP. However, little is known about the regulation of nuclear export of pre-miRNAs in the DNA-damage response, a critical step in miRNA maturation. Here, we show that nuclear export of pre-miRNAs is accelerated after DNA damage in an ATM-dependent manner. The ATM-activated AKT kinase phosphorylates Nup153, a key component of the nucleopore, leading to enhanced interaction between Nup153 and Exportin-5 (XPO5) and increased nuclear export of pre-miRNAs. These findings define an important role of DNA-damage signaling in miRNA transport and maturation.

Rights and Permissions

Citation: Wan G, Zhang X, Langley RR, Liu Y, Hu X, Han C, Peng G, Ellis LM, Jones SN, Lu X. DNA-damage-induced nuclear export of precursor microRNAs is regulated by the ATM-AKT pathway. Cell Rep. 2013 Jun 27;3(6):2100-12. doi: 10.1016/j.celrep.2013.05.038. Link to article on publisher's site

Comments

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

Related Resources

Link to Article in PubMed