Stephen Jones Lab Publications

Title

Coxsackievirus and adenovirus receptor is essential for cardiomyocyte development

UMMS Affiliation

Department of Cell Biology; Department of Medicine

Date

5-3-2005

Document Type

Article

Medical Subject Headings

Animals; Base Sequence; Chimera; Fetus; Gene Expression Regulation, Developmental; Genotype; Heart; Heart Defects, Congenital; In Situ Hybridization; Mice; Mice, Knockout; Molecular Sequence Data; Myocytes, Cardiac; RNA, Messenger; Receptors, Virus

Disciplines

Cell Biology

Abstract

The coxsackievirus and adenovirus receptor (CAR) is a transmembrane protein that is known to be a site of viral attachment and entry, but its physiologic functions are undefined. CAR expression is maximal in neonates and wanes rapidly after birth in organs such as heart, muscle, and brain, suggesting that CAR plays a role in the development of these tissues. Here, we show that CAR deficiency resulted in an embryonic lethal condition associated with cardiac defects. Specifically, commencing approximately 10.5 days postconception (dpc), CAR-/- cardiomyocytes exhibited regional apoptosis evidenced by both histopathologic features of cell death and positive staining for the apoptotic marker cleaved caspase 3. CAR-/- fetuses invariably suffered from degeneration of the myocardial wall and thoracic hemorrhaging, leading to death by 11.5 dpc. These findings are consistent with the view that CAR provides positive survival signals to cardiomyocytes that are essential for normal heart development.

Rights and Permissions

Citation: Genesis. 2005 Jun;42(2):77-85. Link to article on publisher's site

Related Resources

Link to Article in PubMed