UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyProgram in Molecular Medicine
Department of Cell Biology
Information Services, Academic Computing Services
Document Type
Journal ArticlePublication Date
2005-10-28Keywords
DNA ReplicationDNA, Fungal
Genome, Fungal
Replication Origin
S Phase
Schizosaccharomyces
Schizosaccharomyces pombe Proteins
Stochastic Processes
Molecular Biology
Metadata
Show full item recordAbstract
DNA replication initiates at discrete origins along eukaryotic chromosomes. However, in most organisms, origin firing is not efficient; a specific origin will fire in some but not all cell cycles. This observation raises the question of how individual origins are selected to fire and whether origin firing is globally coordinated to ensure an even distribution of replication initiation across the genome. We have addressed these questions by determining the location of firing origins on individual fission yeast DNA molecules using DNA combing. We show that the firing of replication origins is stochastic, leading to a random distribution of replication initiation. Furthermore, origin firing is independent between cell cycles; there is no epigenetic mechanism causing an origin that fires in one cell cycle to preferentially fire in the next. Thus, the fission yeast strategy for the initiation of replication is different from models of eukaryotic replication that propose coordinated origin firing.Source
Mol Biol Cell. 2006 Jan;17(1):308-16. Epub 2005 Oct 26. Link to article on publisher's siteDOI
10.1091/mbc.E05-07-0657Permanent Link to this Item
http://hdl.handle.net/20.500.14038/35323PubMed ID
16251353Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1091/mbc.E05-07-0657