Regulation of Drosophila p38 activation by specific MAP2 kinase and MAP3 kinase in response to different stimuli
Department of Medicine, Division of Infectious Diseases and Immunology
Animals; Cell Line; Drosophila; Heat; MAP Kinase Kinase 1; MAP Kinase Kinase 2; MAP Kinase Kinase 3; MAP Kinase Kinase Kinase 5; MAP Kinase Signaling System; Peptidoglycan; RNA Interference; RNA, Double-Stranded; Signal Transduction; Sodium Chloride; Ultraviolet Rays; p38 Mitogen-Activated Protein Kinases; effects
The p38 mitogen-activated protein kinase (MAPK) signaling pathway plays an important role in cellular responses to inflammatory stimuli and environmental stress. Activation of p38 is mediated through phosphorylation by upstream MAPKK, which in turn is activated by MAPKKK. However, the mechanism of how different upstream MAP2Ks and MAP3Ks specifically contribute to p38 activation in response to different stimuli is still not clearly understood. By using double-stranded RNA-mediated interference (RNAi) in Drosophila cells, we demonstrate that D-MKK3 is a major MAP2K responsible for D-p38 activation by UV, heat shock, NaCl or peptiodglycan (PGN). Stimulation of UV and PGN activates D-p38 through D-MEKK1, heat shock-induced activation of D-p38 signals through both D-MEKK1 and D-ASK1. On the other hand, maximal activation of D-p38 by NaCl requires the expression of four MAP3Ks.
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Citation: Cell Signal. 2006 Apr;18(4):441-8. Epub 2005 Jul 12. Link to article on publisher's site