Human cytotoxic T lymphocyte responses to live attenuated 17D yellow fever vaccine: identification of HLA-B35-restricted CTL epitopes on nonstructural proteins NS1, NS2b, NS3, and the structural protein E
UMass Chan Affiliations
Department of Medicine, Division of Infectious Diseases and ImmunologyCenter for Infectious Disease and Vaccine Research
Document Type
Journal ArticlePublication Date
2002-02-01Keywords
ImmunityImmunology and Infectious Disease
Immunology of Infectious Disease
Infectious Disease
Virology
Metadata
Show full item recordAbstract
Yellow fever virus (YFV) is a re-emerging problem despite the existence of an effective live-attenuated vaccine. The induction of YFV-neutralizing antibodies undoubtedly contributes to vaccine efficacy, but T lymphocyte responses to YFV likely play a role in long-term efficacy. We studied the T lymphocyte responses to YFV in four vaccinees. Proliferation and cytolytic responses to YFV were demonstrated in all subjects. We isolated 13 YFV-specific CD8(+) CTL lines that recognized epitopes on the E, NS1, NS2b, and NS3 proteins; eight CTL lines were HLA-B35-restricted. YFV-specific T cell responses were detectable by IFN gamma ELISPOT assays 14 days postvaccination, with T cell frequencies sustained for up to 19 months. To our knowledge, this is the first report of human T lymphocyte responses following YFV vaccination. These results indicate that the live 17D YFV vaccine induced CD8(+) T cell responses directed against at least four different HLA-B35-restricted YFV epitopes.Source
Virology. 2002 Feb 1;293(1):151-63. doi:10.1006/viro.2001.1255 Link to article on publisher's siteDOI
10.1006/viro.2001.1255Permanent Link to this Item
http://hdl.handle.net/20.500.14038/35071PubMed ID
11853408Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1006/viro.2001.1255