Human metapneumovirus reinfection among children in Thailand determined by ELISA using purified soluble fusion protein
Authors
Pavlin, Julie A.Hickey, Andrew C.
Ulbrandt, Nancy
Chan, Yee-Peng
Endy, Timothy P.
Boukhvalova, Marina S.
Chunsuttiwat, Supamit
Nisalak, Ananda
Libraty, Daniel H.
Green, Sharone
Rothman, Alan L.
Ennis, Francis A.
Jarman, Richard
Gibbons, Robert V.
Broder, Christopher C.
UMass Chan Affiliations
Center for Infectious Disease and Vaccine ResearchDepartment of Medicine, Division of Infectious Diseases and Immunology
Document Type
Journal ArticlePublication Date
2008-09-15Keywords
ImmunityImmunology and Infectious Disease
Immunology of Infectious Disease
Infectious Disease
Metadata
Show full item recordAbstract
BACKGROUND: Human metapneumovirus (hMPV) is a newly discovered paramyxovirus that causes acute respiratory illness. Despite apparent near-universal exposure during early childhood, immunity is transient. METHODS: An indirect screening enzyme-linked immunosorbent assay using a recombinant soluble fusion (F) glycoprotein derived from hMPV was used to test for anti-F IgG in 1,380 pairs of acute- and convalescent-stage serum samples collected from children in Kamphaeng Phet, Thailand. RESULTS: Of the 1,380 serum sample pairs tested, 1,376 (99.7%) showed evidence of prior infection with hMPV. Sixty-six paired specimens demonstrated a > or=4-fold rise in titer, for an overall reinfection rate of 4.9%. Two children demonstrated evidence of an initial infection. Forty-eight of the 68 new infections or reinfections occurred in 2000, accounting for 13.2% of all nonflaviviral febrile illnesses in the study population in that year. Of 68 positive cases, 85.3% complained of cough and 66.2% complained of rhinorrhea, compared with 61.4% and 49.0% of negative cases, respectively (P < .01). All positive samples were also tested for an increase in titer of antibodies to respiratory syncytial virus F, and 27% exhibited a > or=4-fold rise. CONCLUSION: These results demonstrate that hMPV reinfections cause illness at a rate equal to that seen for initial infections. hMPV may have a more significant impact in older children than previously realized and may be the cause of significant outbreaks in this population.Source
J Infect Dis. 2008 Sep 15;198(6):836-42. doi: 10.1086/591186. Link to article on publisher's siteDOI
10.1086/591186Permanent Link to this Item
http://hdl.handle.net/20.500.14038/35031PubMed ID
18680407Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1086/591186