ADA1, a novel component of the ADA/GCN5 complex, has broader effects than GCN5, ADA2, or ADA3
Department of Medicine, Division of Infectious Diseases and Immunology
Acetylation; Blotting, Western; Cloning, Molecular; DNA, Fungal; DNA-Binding Proteins; Fungal Proteins; Histone Acetyltransferases; Histones; Inositol; Macromolecular Substances; Molecular Sequence Data; Phenotype; Protein Kinases; Saccharomyces cerevisiae; *Saccharomyces cerevisiae Proteins; Sequence Analysis, DNA; Trans-Activators; Transcription Factors; Transcription, Genetic
The ADA genes encode factors which are proposed to function as transcriptional coactivators. Here we describe the cloning, sequencing, and initial characterization of a novel ADA gene, ADA1. Similar to the previously isolated ada mutants, ada1 mutants display decreases in transcription from various reporters. Furthermore, ADA1 interacts with the other ADAs in the ADA/GCN5 complex as demonstrated by partial purification of the complex and immunoprecipitation experiments. We estimate that the complex has a molecular mass of approximately 2 MDa. Previously, it had been demonstrated that ada5 mutants displayed more severe phenotypic defects than the other ada mutants (G. A. Marcus, J. Horiuchi, N. Silverman, and L. Guarente, Mol. Cell. Biol. 16:3197-3205, 1996; S. M. Roberts and F. Winston, Mol. Cell. Biol. 16:3206-3213, 1996). ada1 mutants display defects similar to those of ada5 mutants and different from those of the other mutants with respect to promoters affected, inositol auxotrophy, and Spt- phenotypes. Thus, the ADAs can be separated into two classes, suggesting that the ADA/GCN5 complex may have two separate functions. We present a speculative model on the possible roles of the ADA/GCN5 complex.
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Citation: Mol Cell Biol. 1997 Jun;17(6):3220-8. Link to article on publisher's site