UMMS Affiliation

Department of Medicine, Division of Infectious Diseases and Immunology; Program in Molecular Medicine; RNA Therapeutics Institute

Date

6-25-2014

Document Type

Article

Abstract

Virus-host interactions drive a remarkable diversity of immune responses and countermeasures. We found that two RNA viruses with broad host ranges, vesicular stomatitis virus (VSV) and Sindbis virus (SINV), are completely restricted in their replication after entry into Lepidopteran cells. This restriction is overcome when cells are co-infected with vaccinia virus (VACV), a vertebrate DNA virus. Using RNAi screening, we show that Lepidopteran RNAi, Nuclear Factor-kappaB, and ubiquitin-proteasome pathways restrict RNA virus infection. Surprisingly, a highly conserved, uncharacterized VACV protein, A51R, can partially overcome this virus restriction. We show that A51R is also critical for VACV replication in vertebrate cells and for pathogenesis in mice. Interestingly, A51R colocalizes with, and stabilizes, host microtubules and also associates with ubiquitin. We show that A51R promotes viral protein stability, possibly by preventing ubiquitin-dependent targeting of viral proteins for destruction. Importantly, our studies reveal exciting new opportunities to study virus-host interactions in experimentally-tractable Lepidopteran systems.

Rights and Permissions

Citation: Elife. 2014 Jun 25;3. doi: 10.7554/eLife.02910. Link to article on publisher's site

Comments

This is an open-access article, free of all copyright, and may befreely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under theCreative Commons CC0 public domain dedication.

Related Resources

Link to Article in PubMed

Keywords

Immunology, Microbiology and infectious disease, Lymantria dispar, vaccinia virus, vesicular stomatitis virus, Sindbis virus, microtubules, ubiquitin, Mouse Viruses

PubMed ID

24966209

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