RNA and beta-hemolysin of Group B streptococcus induce IL-1beta by activating NLRP3 inflammasomes in mouse macrophages
Department of Medicine, Division of Infectious Diseases and Immunology
The inflammatory cytokine IL-1beta is critical for host responses against many human pathogens. Here, we define Group B streptococcus (GBS)-mediated activation of the Nod-like Receptor-P3 (NLRP3) inflammasome in macrophages. NLRP3 activation requires GBS expression of the cytolytic toxin, beta-hemolysin, lysosomal acidification, and leakage. These processes allow the interaction of GBS RNA with cytosolic NLRP3. The present study supports a model in which GBS RNA, along with lysosomal components including cathepsins, leaks out of lysosomes and interacts with NLRP3 to induce IL-1beta production.
Cell signaling, Immunology, Innate immunity, Interleukin, RNA
Gupta, Rahul; Ghosh, Shubhendu; Monks, Brian G.; DeOliveira, Rosane B.; Tzeng, TeChen; Kalantari, Parisa; Nandy, Anubhab; Bhattacharjee, Bornali; Chan, Jennie; Ferreira, Fabianno; Rathinam, Vijay A.K.; Sharma, Shrutie; Lien, Egil; Silverman, Neal S.; Fitzgerald, Katherine A.; Firon, Arnaud; Trieu-Cuot, Patrick; Henneke, Philipp; and Golenbock, Douglas T., "RNA and beta-hemolysin of Group B streptococcus induce IL-1beta by activating NLRP3 inflammasomes in mouse macrophages" (2014). Infectious Diseases and Immunology Publications and Presentations. Paper 158.