Characterization of poxvirus-encoded proteins that regulate innate immune signaling pathways
Authors
Rus, FlorentinaMorlock, Kayla
Silverman, Neal S.
Pham, Ngoc
Kotwal, Girish J.
Marshall, William L.
UMass Chan Affiliations
Department of Medicine, Division of Infectious Diseases and ImmunologyDocument Type
Journal ArticlePublication Date
2012-06-13Keywords
Amino Acid SequenceApoptosis
Cloning, Molecular
Flow Cytometry
Genes, Reporter
HEK293 Cells
HeLa Cells
*Host-Pathogen Interactions
Humans
Immune Evasion
*Immunity, Innate
Immunomodulation
Immunoprecipitation
Luciferases, Firefly
Luciferases, Renilla
Molecular Sequence Data
Phylogeny
Protein Binding
*Signal Transduction
Toll-Like Receptors
Vaccinia virus
Viral Proteins
Biochemistry, Biophysics, and Structural Biology
Bioinformatics
Immunology and Infectious Disease
Microbiology
Metadata
Show full item recordAbstract
Innate immune recognition of pathogens is critical to the prompt control of infections, permitting the host to survive to develop long-term immunity via an adaptive immune response. Poxviruses encode a family of proteins that inhibit signaling by Toll-like receptors to their downstream signaling components, severely limiting nuclear translocation of transcription factors such as IRF3 and NF-kappaB and thereby decreasing production of host interferons and cytokines. We describe bioinformatics techniques for identifying candidate poxviral inhibitors of the innate immune response based on similarity to the family of proteins that includes A52, A46, and N1. Robust luciferase assays can determine whether a given poxviral gene affects innate immune signaling, and in combination with other approaches can identify the cellular targets of poxviral innate immune evasion genes. Because apoptosis is an innate immune response of the cell to viral infection, assays for identifying poxviral genes that inhibit apoptosis can also be employed. Novel poxviral innate immune inhibitors are being identified via several approaches and these techniques promise to identify further complexities in the way that poxviruses interact with the host innate immune system.Source
Methods Mol Biol. 2012;890:273-88. doi: 10.1007/978-1-61779-876-4_16. Link to article on publisher's siteDOI
10.1007/978-1-61779-876-4_16Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34927PubMed ID
22688773Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1007/978-1-61779-876-4_16