TRIF licenses caspase-11-dependent NLRP3 inflammasome activation by gram-negative bacteria
Department of Medicine, Division of Infectious Diseases and Immunology
Adaptor Proteins, Vesicular Transport; Animals; Carrier Proteins; Caspases; Citrobacter rodentium; Enterohemorrhagic Escherichia coli; Gram-Negative Bacteria; Gram-Positive Bacteria; Inflammasomes; Interferons; Mice; Signal Transduction
Systemic infections with Gram-negative bacteria are characterized by high mortality rates due to the "sepsis syndrome," a widespread and uncontrolled inflammatory response. Though it is well recognized that the immune response during Gram-negative bacterial infection is initiated after the recognition of endotoxin by Toll-like receptor 4, the molecular mechanisms underlying the detrimental inflammatory response during Gram-negative bacteremia remain poorly defined. Here, we identify a TRIF pathway that licenses NLRP3 inflammasome activation by all Gram-negative bacteria. By engaging TRIF, Gram-negative bacteria activate caspase-11. TRIF activates caspase-11 via type I IFN signaling, an event that is both necessary and sufficient for caspase-11 induction and autoactivation. Caspase-11 subsequently synergizes with the assembled NLRP3 inflammasome to regulate caspase-1 activation and leads to caspase-1-independent cell death. These events occur specifically during infection with Gram-negative, but not Gram-positive, bacteria. The identification of TRIF as a regulator of caspase-11 underscores the importance of TLRs as master regulators of inflammasomes during Gram-negative bacterial infection.
Rathinam, Vijay A. K.; Vanaja, Sivapriya Kailasan; Waggoner, Lisa; Sokolovska, Anna; Becker, Christine; Stuart, Lynda M.; Leong, John M.; and Fitzgerald, Katherine A., "TRIF licenses caspase-11-dependent NLRP3 inflammasome activation by gram-negative bacteria" (2012). Infectious Diseases and Immunology Publications and Presentations. 143.