Title

Activation of autophagy by inflammatory signals limits IL-1beta production by targeting ubiquitinated inflammasomes for destruction

UMMS Affiliation

Department of Medicine, Division of Infectious Diseases and Immunology

Date

1-29-2012

Document Type

Article

Subjects

Animals; *Autophagy; Carrier Proteins; Cell Line; Humans; Inflammasomes; Inflammation; Interleukin-1beta; Mice; Nuclear Proteins; *Signal Transduction; *Ubiquitination; ral GTP-Binding Proteins

Abstract

Autophagosomes delivers cytoplasmic constituents to lysosomes for degradation, whereas inflammasomes are molecular platforms activated by infection or stress that regulate the activity of caspase-1 and the maturation of interleukin 1beta (IL-1beta) and IL-18. Here we show that the induction of AIM2 or NLRP3 inflammasomes in macrophages triggered activation of the G protein RalB and autophagosome formation. The induction of autophagy did not depend on the adaptor ASC or capase-1 but was dependent on the presence of the inflammasome sensor. Blocking autophagy potentiated inflammasome activity, whereas stimulating autophagy limited it. Assembled inflammasomes underwent ubiquitination and recruited the autophagic adaptor p62, which assisted their delivery to autophagosomes. Our data indicate that autophagy accompanies inflammasome activation to temper inflammation by eliminating active inflammasomes.

Comments

Citation: Nat Immunol. 2012 Jan 29;13(3):255-63. doi: 10.1038/ni.2215. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

22286270