Activation of autophagy by inflammatory signals limits IL-1beta production by targeting ubiquitinated inflammasomes for destruction
Department of Medicine, Division of Infectious Diseases and Immunology
Animals; *Autophagy; Carrier Proteins; Cell Line; Humans; Inflammasomes; Inflammation; Interleukin-1beta; Mice; Nuclear Proteins; *Signal Transduction; *Ubiquitination; ral GTP-Binding Proteins
Autophagosomes delivers cytoplasmic constituents to lysosomes for degradation, whereas inflammasomes are molecular platforms activated by infection or stress that regulate the activity of caspase-1 and the maturation of interleukin 1beta (IL-1beta) and IL-18. Here we show that the induction of AIM2 or NLRP3 inflammasomes in macrophages triggered activation of the G protein RalB and autophagosome formation. The induction of autophagy did not depend on the adaptor ASC or capase-1 but was dependent on the presence of the inflammasome sensor. Blocking autophagy potentiated inflammasome activity, whereas stimulating autophagy limited it. Assembled inflammasomes underwent ubiquitination and recruited the autophagic adaptor p62, which assisted their delivery to autophagosomes. Our data indicate that autophagy accompanies inflammasome activation to temper inflammation by eliminating active inflammasomes.
Shi, Chong-Shan; Shenderov, Kevin; Huang, Ning-Na; Kabat, Juraj; Mones, Abu-Asab; Fitzgerald, Katherine A.; Sher, Alan; and Kehrl, John H., "Activation of autophagy by inflammatory signals limits IL-1beta production by targeting ubiquitinated inflammasomes for destruction" (2012). Infectious Diseases and Immunology Publications and Presentations. Paper 134.