Bacteriophage P22 accessory recombination function
Graduate School of Biomedical Sciences; Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology
Medical Subject Headings
Amino Acid Sequence; Bacteriophage lambda; Base Sequence; DNA Mutational Analysis; DNA, Viral; *Genes, Viral; Molecular Sequence Data; *Recombination, Genetic; Salmonella Phages; Viral Proteins; Viral Structural Proteins; Virus Replication
Life Sciences | Medicine and Health Sciences
The accessory recombination function (arf) gene of bacteriophage P22 is located immediately upstream of the essential recombination function (erf) gene. Three mutant alleles of arf were constructed and installed in P22 in place of the wild-type allele: an out-of-frame internal deletion, an in-frame internal deletion, and an amber mutation. The deletion mutant phages are partially defective in homologous recombination and plaque formation in wild-type and recA hosts; their defects are more severe in recB and recA recB hosts. The amber mutant phage exhibits the same growth phenotypes in nonsuppressing hosts, but not in an amber-suppressor host. Plasmids that express arf complement the growth defect of arf- phages. These plasmids stimulate erf-mediated recombination; they were also found to cause a small stimulation of recA-recBCD-mediated homologous recombination of phage lambda.
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Citation: Virology. 1991 May;182(1):316-23.
Poteete, Anthony R.; Fenton, Anita C.; and Semerjian, Arlene, "Bacteriophage P22 accessory recombination function" (1991). GSBS Student Publications. 999.