GSBS Student Publications

Title

Bacteriophage P22 accessory recombination function

UMMS Affiliation

Graduate School of Biomedical Sciences; Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology

Date

5-1-1991

Document Type

Article

Medical Subject Headings

Amino Acid Sequence; Bacteriophage lambda; Base Sequence; DNA Mutational Analysis; DNA, Viral; *Genes, Viral; Molecular Sequence Data; *Recombination, Genetic; Salmonella Phages; Viral Proteins; Viral Structural Proteins; Virus Replication

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The accessory recombination function (arf) gene of bacteriophage P22 is located immediately upstream of the essential recombination function (erf) gene. Three mutant alleles of arf were constructed and installed in P22 in place of the wild-type allele: an out-of-frame internal deletion, an in-frame internal deletion, and an amber mutation. The deletion mutant phages are partially defective in homologous recombination and plaque formation in wild-type and recA hosts; their defects are more severe in recB and recA recB hosts. The amber mutant phage exhibits the same growth phenotypes in nonsuppressing hosts, but not in an amber-suppressor host. Plasmids that express arf complement the growth defect of arf- phages. These plasmids stimulate erf-mediated recombination; they were also found to cause a small stimulation of recA-recBCD-mediated homologous recombination of phage lambda.

Rights and Permissions

Citation: Virology. 1991 May;182(1):316-23.

Related Resources

Link to article in PubMed

Journal Title

Virology

PubMed ID

1827223