The virus-specific and allospecific cytotoxic T-lymphocyte response to lymphocytic choriomeningitis virus is modified in a subpopulation of CD8(+) T cells coexpressing the inhibitory major histocompatibility complex class I receptor Ly49G2
Graduate School of Biomedical Sciences; Program in Immunology and Virology; Department of Pathology
Medical Subject Headings
Animals; *Antigens, Ly; Cells, Cultured; *Complementarity Determining Regions; Cytotoxicity Tests, Immunologic; Flow Cytometry; H-2 Antigens; Immunoglobulin Variable Region; Lectins, C-Type; Lymphocyte Activation; Lymphocytic Choriomeningitis; Lymphocytic choriomeningitis virus; Membrane Glycoproteins; Mice; Mice, Inbred C57BL; Receptors, Antigen, T-Cell; T-Lymphocyte Subsets; T-Lymphocytes, Cytotoxic
Life Sciences | Medicine and Health Sciences
The role of negatively signaling NK cell receptors of the Ly49 family on the specificity of the acute CD8(+) cytotoxic T-lymphocyte (CTL) response was investigated in lymphocytic choriomeningitis virus (LCMV)-infected C57BL/6 mice. Activated CD8(+) T cells coexpressing Ly49G2 expanded during LCMV infection, and T-cell receptor analyses by flow cytometry and CDR3 spectratyping revealed a unique polyclonal T-cell population in the Ly49G2(+) fraction. These cells lysed syngeneic targets infected with LCMV or coated with two of three LCMV immunodominant peptides examined. Transfection of these sensitive targets with H2D(d), a ligand for Ly49G2, inhibited lysis. This was reversed by antibody to Ly49G2, indicating effective negative signaling. LCMV characteristically induces an anti-H2(d) allospecific T-cell response that includes T-cell clones cross-reactive between allogeneic and LCMV-infected syngeneic targets. The CD8(+) Ly49G2(+) population mediated no allospecific killing, nor was any NK-like killing observed against YAC-1 cells. This study shows that CD8(+) Ly49G2(+) cells participate in the virus-induced CTL response but lyse a more restricted range of targets than the rest of the virus-induced CTL population.
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Citation: J Virol. 2000 Aug;74(15):7032-8.
Journal of virology
Peacock, Craig D.; Lin, Meei-Yun; Ortaldo, John R.; and Welsh, Raymond M., "The virus-specific and allospecific cytotoxic T-lymphocyte response to lymphocytic choriomeningitis virus is modified in a subpopulation of CD8(+) T cells coexpressing the inhibitory major histocompatibility complex class I receptor Ly49G2" (2000). GSBS Student Publications. 971.