Isolated flagellar outer arm dynein translocates brain microtubules in vitro
Authors
Paschal, Bryce MarkKing, Stephen M.
Moss, Anthony G.
Collins, Christine A.
Vallee, Richard B.
Witman, George B.
Document Type
Journal ArticlePublication Date
1987-12-17Keywords
Adenosine Triphosphatases; Animals; Brain; Dynein ATPase; Flagella; Microtubules; Movement; Sea Urchins; Video RecordingLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
The inner and outer arms of the flagellar axoneme generate the forces needed for flagellar movement; these arms contain ATPases called dyneins. To date, there has been no method for studying the mechanochemical transducing activity of isolated dyneins. Recently, it was found that the brain microtubule-associated protein (MAP) 1C is a microtubule-activated ATPase with the structural and force-producing properties of dynein. MAP 1C translocates microtubules in an in vitro gliding assay, suggesting that such an assay could also be used with axonemal dyneins. Here, we demonstrate that outer-arm dynein isolated from sea urchin (Strongylocentrotus purpuratus) sperm and adsorbed to a glass coverslip can translocate calf-brain microtubules along the surface of the coverslip. Our results conclusively demonstrate that outer-arm dynein by itself is capable of generating shearing forces. The ability to examine the force-generating properties of flagellar dynein in vitro should greatly facilitate studies of the mechanism of action of this important mechanochemical transducer.Source
Nature. 1987 Dec 17-23;330(6149):672-4. Link to article on publisher's siteDOI
10.1038/330672a0Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34314PubMed ID
2960903Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1038/330672a0