GSBS Student Publications

Title

A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology

Date

12-20-1988

Document Type

Article

Medical Subject Headings

*Chromosome Deletion; Chromosome Mapping; Chromosomes, Bacterial; Crosses, Genetic; *DNA Transposable Elements; Escherichia coli; *Genes; *Genes, Bacterial; Genotype; *Mutation; Site-Specific DNA-Methyltransferase (Adenine-Specific); Transduction, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We describe the isolation of a strain of Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the dam gene (dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of E. coli. The substitution mutation confers resistance to kanamycin and can easily be transferred to other strains by standard genetic techniques. The amount of Dam methyltransferase (MTase) in dam-16 strains as determined either in vitro or in vivo is below the level of detection. We conclude that the Dam MTase is not required for viability of E. coli.

Rights and Permissions

Citation: Gene. 1988 Dec 20;73(2):531-5.

Related Resources

Link to article in PubMed

Journal Title

Gene

PubMed ID

2854098