Mutational destabilization of the critical interface water cluster in Scapharca dimeric hemoglobin: structural basis for altered allosteric activity
Graduate School of Biomedical Sciences; Program in Molecular Medicine; Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Allosteric Regulation; Animals; Bivalvia; Carbon Monoxide; Crystallography, X-Ray; Dimerization; Hemoglobins; Hydrogen Bonding; Models, Molecular; Molecular Sequence Data; Protein Conformation; Water
Life Sciences | Medicine and Health Sciences
A cluster of interface ordered water molecules has been proposed to act as a key mediator of intersubunit communication in the homodimeric hemoglobin of Scapharca inaequivalvis. Mutations of Thr72 to Val and Ile, which lack the hydroxyl group to hydrogen bond the deoxy interface water molecules, result in sharply altered functional properties. We have determined the high resolution (1.6-1. 8 A) crystal structures of these two mutants in both the deoxygenated and CO-liganded states. These structures show minimal protein structural changes relative to the same native derivatives, despite greater than 40-fold increases in oxygen affinity. In the deoxy state of both mutants two water molecules at the periphery of the water cluster are lost, and the remaining cluster water molecules are destabilized. The CO-liganded structures show key differences between the two mutants including a more optimal interface packing involving Ile72 that acts to stabilize its high affinity (R) state. This additional stabilization allows rationalization of its lowered cooperativity within the context of a two-state model. These studies support a key role of ordered water in cooperative functioning and illustrate how subtle structural alterations can result in significantly altered functional properties in an allosteric molecule.
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Citation: J Mol Biol. 1998 Dec 4;284(3):729-39. Link to article on publisher's site
Journal of molecular biology
Pardanani, Animesh Dev; Gambacurta, Alessandra; Ascoli, Franca; and Royer, William E., "Mutational destabilization of the critical interface water cluster in Scapharca dimeric hemoglobin: structural basis for altered allosteric activity" (1998). GSBS Student Publications. 955.