GSBS Student Publications

Title

Cellular localization of human Rad51C and regulation of ubiquitin-mediated proteolysis of Rad51

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology

Date

10-11-2005

Document Type

Article

Medical Subject Headings

Animals; Cells, Cultured; DNA Damage; DNA Repair; DNA-Binding Proteins; Gene Expression Regulation; Hela Cells; Humans; Microscopy, Confocal; RNA, Small Interfering; Rad51 Recombinase; Ubiquitin

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Rad51-catalyzed homologous recombination is an important pathway for repair of DNA double strand breaks and maintenance of genome integrity in vertebrate cells. Five proteins referred to as Rad51 paralogs promote Rad51 activity and are proposed to act at various, and in some cases, multiple stages in the recombination pathway. Imaging studies of native Rad51 have revealed its cellular response to DNA damage, yet visualization of the paralog proteins has met with limited success. In this study, we are able to detect endogenous Rad51C and Xrcc3 in human cells. In an effort to determine how Rad51, Rad51C, and Xrcc3 influence the pattern of localization of each other over the time course of DNA damage and repair, we have made the unexpected observation that Rad51 degradation via the ubiquitin-mediated proteasome pathway occurs as a natural part of recombinational DNA repair. Additionally, we find that Rad51C plays an important role in regulating this process. This article contains supplementary material, which may be viewed at the Journal of Cellular Biochemistry website at http://www.interscience.wiley.com/jpages/0730-2312/suppmat/index.html.

Rights and Permissions

Citation: J Cell Biochem. 2005 Dec 15;96(6):1095-109. Link to article on publisher's site

DOI of Published Version

10.1002/jcb.20640

Related Resources

Link to article in PubMed

Journal Title

Journal of cellular biochemistry

PubMed ID

16215984