CPEB-mediated cytoplasmic polyadenylation and the regulation of experience-dependent translation of alpha-CaMKII mRNA at synapses
Authors
Wu, LinWells, David G.
Tay, Joyce
Mendis, Duane
Abbott, Mary-Alice
Barnitt, Allan
Quinlan, Elizabeth
Heynen, Arnold
Fallon, Justin R.
Richter, Joel D.
UMass Chan Affiliations
Program in Molecular MedicineDepartment of Molecular Genetics and Microbiology
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
1998-12-18Keywords
Animals; Brain; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Calcium-Calmodulin-Dependent Protein Kinases; Cells, Cultured; Cytoplasm; Hippocampus; Mice; Neurons; Oocytes; Organ Specificity; RNA, Messenger; RNA-Binding Proteins; Rats; Rats, Long-Evans; Rats, Wistar; Synapses; Transcription Factors; Xenopus; *Xenopus Proteins; *mRNA Cleavage and Polyadenylation FactorsLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Long-term changes in synaptic efficacy may require the regulated translation of dendritic mRNAs. While the basis of such regulation is unknown, it seemed possible that some features of translational control in development could be recapitulated in neurons. Polyadenylation-induced translation of oocyte mRNAs requires the cis-acting CPE sequence and the CPE-binding protein CPEB. CPEB is also present in the dendritic layers of the hippocampus, at synapses in cultured neurons, and in postsynaptic densities of adult brain. alpha-CaMKII mRNA, which is localized in dendrites and is necessary for synaptic plasticity and LTP, contains two CPEs. These CPEs are bound by CPEB and mediate polyadenylation-induced translation in injected Xenopus oocytes. In the intact brain, visual experience induces alpha-CaMKII mRNA polyadenylation and translation, suggesting that this process likely occurs at synapses.Source
Neuron. 1998 Nov;21(5):1129-39.
DOI
https://escholarship.umassmed.edu/gsbs_sp/949Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34297PubMed ID
9856468Related Resources
ae974a485f413a2113503eed53cd6c53
https://escholarship.umassmed.edu/gsbs_sp/949