GSBS Student Publications

Title

Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology; Program in Molecular Medicine

Date

4-6-2004

Document Type

Article

Medical Subject Headings

Adenylate Kinase; Amino Acid Sequence; Animals; Base Sequence; *Chlamydomonas reinhardtii; Cloning, Molecular; Dynein ATPase; Flagella; Genes, Protozoan; Molecular Sequence Data; Mutagenesis, Insertional; Mutation; Protein Binding; Protozoan Proteins; RNA, Messenger

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Of the uncloned ODA genes required for outer dynein arm assembly in Chlamydomonas, ODA5 and ODA10 are of particular interest because they do not encode known subunits of the outer arm or the outer dynein arm-docking complex (ODA-DC), and because genetic studies suggest their products interact. Beginning with a tagged oda5 allele, we isolated genomic and cDNA clones of the wild-type gene. ODA5 predicts a novel, 66-kDa coiled-coil protein. Immunoblotting indicates Oda5p is an axonemal component that assembles onto the axoneme independently of the outer arm and ODA-DC and is uniquely missing in oda5 and oda10 axonemes. Oda5p is released from the axoneme by extraction with 0.6 M KCl, but the soluble Oda5p does not cosediment with the outer dynein arm/ODA-DC in sucrose gradients. Quantitative mass spectrometry by using isotope coded affinity tagging revealed that a previously unidentified adenylate kinase is reduced 35-50% in oda5 flagella. Direct enzymatic assays demonstrated a comparable reduction in adenylate kinase activity in oda5 flagella, and also in oda10 flagella, but not in flagella of other oda mutants. We propose that Oda5p is part of a novel axonemal complex that is required for outer arm assembly and anchors adenylate kinase in proximity to the arm.

Rights and Permissions

Citation: Mol Biol Cell. 2004 Jun;15(6):2729-41. Epub 2004 Apr 2. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

Molecular biology of the cell

PubMed ID

15064350