SRC-1 and Wnt signaling act together to specify endoderm and to control cleavage orientation in early C. elegans embryos
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Medical Subject Headings
Animals; Body Patterning; Caenorhabditis elegans; *Caenorhabditis elegans Proteins; Cell Division; Cell Lineage; Cell Polarity; DNA-Binding Proteins; Embryo, Nonmammalian; Endoderm; Gene Expression Regulation, Developmental; Helminth Proteins; High Mobility Group Proteins; Molecular Sequence Data; Phosphotyrosine; Proto-Oncogene Proteins; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Signal Transduction; Wnt Proteins; *Zebrafish Proteins; src-Family Kinases
Life Sciences | Medicine and Health Sciences
In early C. elegans embryos, signaling between a posterior blastomere, P2, and a ventral blastomere, EMS, specifies endoderm and orients the division axis of the EMS cell. Although Wnt signaling contributes to this polarizing interaction, no mutants identified to date abolish P2/EMS signaling. Here, we show that two tyrosine kinase-related genes, src-1 and mes-1, are required for the accumulation of phosphotyrosine between P2 and EMS. Moreover, src-1 and mes-1 mutants strongly enhance endoderm and EMS spindle rotation defects associated with Wnt pathway mutants. SRC-1 and MES-1 signal bidirectionally to control cell fate and division orientation in both EMS and P2. Our findings suggest that Wnt and Src signaling function in parallel to control developmental outcomes within a single responding cell.
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Citation: Dev Cell. 2002 Jul;3(1):113-25.
Bei, Yanxia; Hogan, Jennifer; Berkowitz, Laura A.; Soto, Martha C.; Rocheleau, Christian Ernest; Pang, Ka Ming; Collins, John J.; and Mello, Craig C., "SRC-1 and Wnt signaling act together to specify endoderm and to control cleavage orientation in early C. elegans embryos" (2002). GSBS Student Publications. 93.